ERIC-PCR-based strain-specific detection of phenol-degrading bacteria in activated sludge of wastewater treatment systems
نویسندگان
چکیده
منابع مشابه
Population dynamics of phenol-degrading bacteria in activated sludge determined by gyrB-targeted quantitative PCR.
A method for quantifying bacterial populations introduced into an activated-sludge microbial community is described. The method involves extraction of DNA from activated sludge, appropriate dilution of the extracted DNA with DNA extracted from nonintroduced activated sludge, PCR amplification of a gyrB gene fragment from the introduced strain with a set of strain-specific primers, and quantific...
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Phenol is one of the organic pollutants in various industrial wastewaters especially petrochemical and oil refining. Biological treatment is one of the considerable choices for removing of phenol present in these wastewaters. Identification of effective microbial species is considered as one of the important priorities for production of the biomass in order to achieve desirable kinetic of biolo...
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DNA was isolated from phenol-digesting activated sludge, and partial fragments of the 16S ribosomal DNA (rDNA) and the gene encoding the largest subunit of multicomponent phenol hydroxylase (LmPH) were amplified by PCR. An analysis of the amplified fragments by temperature gradient gel electrophoresis (TGGE) demonstrated that two major 16S rDNA bands (bands R2 and R3) and two major LmPH gene ba...
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Textile effluents are characterised by high content of recalcitrant compounds and are often discharged (together with municipal wastewater to increase their treatability) into centralized wastewater treatment plants with a complex treatment scheme. This paper reports the results achieved adopting a granular sludge system (sequencing batch biofilter granular reactor - SBBGR) to treat mixed munic...
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Linear alkylbenzene sulfonate (LABS) is an anionic surfactant widely used all over the world. They will eventually end-up and accumulate in household or industrial sewage. Due to their high foaming capabilities which can cause numerous problems in sewage treatment facilities as well as direct toxic effects on many different organisms in ecosystem they are generally considered as serious polluta...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2009
ISSN: 0266-8254,1472-765X
DOI: 10.1111/j.1472-765x.2009.02696.x